Induction of Chlorophyll Mutations through Physical and Chemical Mutagenesis in Cowpea [Vigna unguiculata (L.) Walp.]
- Janta Vedic College, Baraut, Baghpat, Uttar Pradesh.(Ch. Charan Singh University, Meerut).
- ICAR-Indian Institute of Wheat & Barley Research, Karnal 132 001 (Haryana).
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Cowpea variety Pusa komal was treated with γ- rays, EMS and combination of gamma rays with EMS to obtain spectrum and frequency of chlorophyll mutations in M2 generation in the present investigation. The individual treatment of EMS was found more efficient than gamma rays to induce chlorophyll mutants. A progressive increase in mutation frequency of chlorophyll mutations was observed with increasing doses. Four different types of induced chlorophyll mutants such as, albino, xantha, chlorina and viridis were observed. The highest frequency of chlorophyll mutants (2.00%) was reported at dose of 200Gy + 0.2% EMS. A dose dependent increase in the spectrum and frequency of chlorophyll mutations was observed whether mutagens were applied singly or in combination.
[Devmani Bind, VK Dwivedi and SK Singh. (2016); Induction of Chlorophyll Mutations through Physical and Chemical Mutagenesis in Cowpea [Vigna unguiculata (L.) Walp.] Int. J. of Adv. Res. 4 (Feb). 49-53] (ISSN 2320-5407). www.journalijar.com
